Biotechnology Principles and Processes Class 12th Chapter 9 Notes PDF NEET 2027 + PYQ
Hello friend, I am NEET Dropper, today I'm sharing complete notes *Chapter 9: Biotechnology - Principles and Processes* 🧬 * SHORT NOTES. NEET 2027* *Note*: New NCERT ✅ ### *1. BIOTECHNOLOGY KE 2 CORE PRINCIPLES* 1. *Genetic Engineering* = Gene ko modify karke host me daalna taaki naya product bane 2. *Bioprocess Engineering* = Microbes/cells ko sterile condition me large scale pe grow karna *rDNA Technology ke 3 Steps*: 1. Identification of DNA with desirable gene 2. Introduction of rDNA into host 3. Maintenance of introduced DNA + Gene expression ### *2. TOOLS OF rDNA TECHNOLOGY* 🔧 #### *A) Restriction Enzymes = Molecular Scissors* ✂️ *Kaam*: DNA ko specific jagah pe cut karte hain *Ex*: _EcoRI_ = _E.coli_ RY13 strain se *Diagram: EcoRI ka Action* 5'---G AATTC---3' 5'---G AATTC---3' 3'---CTTAA G---5' → 3'---CTTAA G---5' ↑ EcoRI site Sticky ends ban gaye *Palindromic Sequence*: 5'-GAATTC-3' | 3'-CTTAAG-5' → Dono side same pad...